Department of Biotechnology
Institute for Biochemistry, Biotechnology and Bioinformatics





Michael Hust
Prof. Dr. Michael Hust

Research group leader

Room: 106
Phone: +49.531.391.5760
Fax: +49.531.391.5763
e-mail: m.hust@tu-bs.de

Research interests

Antibody Engineering:

- recombinant antibodies

- antibody phage display

- antibody gene libraries

- antibodies for diagnostic and therapy with a focus on pathogens on toxins (AntiBotABE, CDiff, DATMAB, LACOP)

- CAR-T cells

Biomarker and vaccines:

- identification of disease related biomarkers (immunogenic oligopeptides) using phage display

- biomarkers for diagnostic of pathogens

- develoment of a tick vaccine (GO-Bio8 project)



 Curriculum vitae
 
10/1993 - 7/1999 Studies of Biology at the Carl-von-Ossietzky Universität, Oldenburg.

4/1998 - 7/1999 Diploma thesis: "Molekularbiologische Untersuchungen zur Diversität von Diatomeenarten und Nachweis der Expressionsraten von Diatomeen fcp Genen im Wattsediment" (Molecularbiological analysis of diatom diversity and investigations on expression rates of the diatom fcp genes in the Wadden Sea sediments) in the AG Geomikrobiologie of the Institut für Chemie und Biologie des Meeres under the supervison of Prof. W.E. Krumbein and PD Dr. Erhard Rhiel.

9/1999 - 7/2002 PhD thesis: "Rekombinante Antikörper gegen die NIa-Protease des Plum Pox Virus" (Recombinant antibodies against the NIa protease of the plum pox virus) at the Lehrgebiet Molekulargenetik (now Institut für Pflanzengenetik, Abteilung Pflanzenbiotechnologie), Universität Hannover (now Leibniz Universität Hannover) under the supervision of Prof. Dr. H.-J. Jacobsen and Dr. Thomas Reinard.

since 12/2002 Group leader at the Institut für Biochemie und Biotechnologie, Abteilung Biotechnologie, Technische Universität Braunschweig headed by Prof. Dr. Stefan Dübel. Lecturer for molecular biotechnology.

5/2011 Habilitation (professorial dissertation, venia legendi for biotechnology), appointed as Privatdozent (PD).

12/2014 Appointed as professor for biotechnology (außerplanmäßiger Professor).


 
Publications


2019
  • (136) Tenspolde, M., Zimmermann, K., Weber, L.C., Hapke, M., Lieber, M., Dywicki, J., Frenzel, A., Hust, M., Galla, M., Buitrago-Molina, L.E., Manns, M.P., Jaeckel, E. and Hardtke-Wolenski, M. (in press). Regulatory T cells engineered with a novel insulin-specific chimeric antigen receptor as a candidate immunotherapy for type 1 diabetes. J. Autoimmunity
  • (135) Russo, G., Fühner, V., Frenzel, A., Hust, M. and Dübel, S. (2019) Parallelized Microscale Expression of Soluble scFv. Metheds Molecular Biology 2025: 203-211
  • (134) Chauvin, D., Hust, M., Schütte, M., Chesnay, A., Parent, C., Moreira, G.M.S.G, Arroyo, J., Sanz, A.B., Pugnière, M., Martineau, P., Chandenier, J., Heuzé-Vourc’h, N.  and Desoubeaux, G. (2019). Targeting Aspergillus fumigatus Crf Transglycosylases With Neutralizing Antibody Is Relevant but Not Sufficient to Erase Fungal Burden in a Neutropenic Rat Model. Frontiers in Microbiology 10: 600
  • (133) Kopertekh, L., Meyer, T., Freyer, C. and Hust M. (2019). Transient plant production of Salmonella Typhimurium diagnostic antibodies. Biotechnology Reports 21:e00314
  • (132) Ramli, R.S., Moreira, G.M.S.G., Zantow, J., Goris, M.G.A., Nguyen, V.K., Novoselova, N., Pessler, F. and Hust, M. (2019). Discovery of Leptospira spp. seroreactive peptides using ORFeome Phage Display. PLOS Neglected Tropical Diseases 13:e0007131
  • (131) Fühner, V., Heine, P.A., Zilkens, K., Meier, D., Roth, K.D.R., Moreira, G.M.S.G., Hust, M. and Russo, G. (2019). Epitope Mapping via Phage Display from single gene libraries. Methods Molecular Biology 1904: 353-375


2018

  • (130) Fühner, V., Heine, P.A., Helmsing, S., Goy, S., Heidepriem, J., Loeffler, F.F., Dübel, S., Gerhard, R. and Hust, M. (2018). Development of Neutralizing and Non-neutralizing Antibodies Targeting Known and Novel Epitopes of TcdB of Clostridioides difficile. Frontiers in Microbiology 9: 2908
  • (129) Chung, S.Y., Schöttelndreier, D., Tatge, H., Fühner, V., Hust, M., Beer, L.A. and Gerhard, R. (2018). The conserved Cys-2232 in Clostridioides difficile toxin B modulates oligomerization and receptor binding. Frontiers in Microbiology 9: 2314
  • (128) Beer, L.A., Tatge, H., Schneider, C., Ruschig, M., Hust, M., Barton, J., Thiemann, S., Fühner, V., Russo, G. and Gerhard, R. (2018). The binary toxin CDT of Clostridium difficile as tool for intracellular delivery of bacterial glucosyltransferase domains. Toxins 10: 225
  • (127) Froude, J.W., Herbert, A.S., Pelat, T., Miethe, S., Zak, S.E., Brannan, J.M., Bakken, R.R., Steiner, A.R., Yin, G., Hallam, T.J., Sato, A.K., Hust, M., Thullier, P. and Dye, J.M. (2018). Post-Exposure Protection in Mice against Sudan Virus by a Two Antibody Cocktail. Viruses 10: 286
  • (126) Burke, C.W., Froude, J.W., Miethe, S., Hülseweh, B., Hust, M. and Glass, P.J. (2018). Human-Like Neutralizing Antibodies Protect Mice from Aerosol Exposure with Western Equine Encephalitis Virus. Viruses 10: 147
  • (125) Sadana, P., Geyer, R., Pezoldt, J., Helmsing, S., Huehn, J., Hust, M., Dersch, P. and Scrima, A. (2018). The invasin D protein from Yersinia pseudotuberculosis selectively binds the Fab region of host antibodies and affects colonization of the intestine. Journal of Biological Chemistry 293: 8672-8690

  • (124) Russo, G., Theisen, U., Fahr, W., Helmsing, S., Hust, M., Köster R.W. and Dübel, S. (2018). Sequencedefined antibodies improve the detection of Cadherin 2 (N-Cadherin) during Zebrafish development. New Biotechnology 45: 98-112
  • (123) Dübel, S., Hust, M., Kügler, J., Kuhn, P., Frenzel, A., Krah, S. and Zielonka, S. (2018). Trendbericht Biochemie 2018: Menschliche Antikörper für Medikamente. Nachrichten aus der Chemie 66: 284-290
  • (122) Moreira, G.M.S.G., Fühner, V. and Hust, M. (2018). Epitope Mapping by Phage Display. Phage display, Ed: Lim, T.S. and Hust, M., Methods Molecular Biology 1701: 497-518
  • (121) Zantow, J., Moreira, G.M.S.G., Dübel, S. and Hust, M. (2018. ORFeome Phage Display. Phage display, Ed: Lim, T.S. and Hust, M., Methods Molecular Biology 1701: 477-495
  • (120) Unkauf, T., Hust, M. and Frenzel, A. (2018). Antibody Affinity and Stability Maturation by Error-Prone PCR. Phage display, Ed: Lim, T.S. and Hust, M., Methods Molecular Biology 1701: 393-407
  • (119) Russo, G., Meier, D., Helmsing, S., Wenzel, E., Oberle, F., Frenzel, A. and Hust, M. (2018). Parallelized antibody selection in microtiter plates. Phage display, Ed: Lim, T.S. and Hust, M., Methods Molecular Biology 1701: 273-284
  • (118) Avril, A., Miethe, S., Hust, M. and Pelat, T. (2018). Construction of macaque immune libraries. Phage display, Ed: Lim, T.S. and Hust, M., Methods Molecular Biology 1701: 83-112
  • (117) Kügler, J., Tomszak, F., Frenzel, A. and Hust, M. (2018). Construction of Human Immune and Naive scFv Libraries. Phage display, Ed: Lim, T.S. and Hust, M., Methods Molecular Biology 1701: 3-24

2017
  • (116) Frenzel, A., Kügler, J., Helmsing, S., Meier, D., Schirrmann, T., Hust, M. and Dübel, S. (2017). Designing human antibodies by phage display. Tranfusion Medicine and Hemotherapy 44: 312-318
  • (115) Rasetti-Escargueil , C., Avril, A., Miethe, S., Mazuet, C., Derman, Y., Selby, K., Thullier, P., Pelat, T., Urbain, R., Fontayne, A., Korkeala, H., Sesardic, D., Hust, M. and Popoff, M.R. (2017) The European AntibotABE Framework Program and Its Update: Development of Innovative Botulinum Antibodies. Toxins 9: 309
  • (114) Stech, M., Nikolaeva, O., Thoring, L., Stöcklein, W.F.M., Wüstenhagen, D.A., Hust, M., Dübel, S. and Kubick, S. (2017). Cell-free synthesis of functional antibodies using a coupled in vitro transcription-translation system based on CHO cell lysates.  Scientific Reports 7:12030
  • (113) Kuhn, P., Thiem, S., Steinert, M., Purvis, D., Lugmayr, V., Treutlein, U., Plobner, L. , Leiser, R.-M., Hust, M. and Dübel, S. (2017). Human Anti-Lipopolysaccharid (LPS) antibodies against Legionella with high species specificity. Human Antibodies 26: 29-38
  • (112) Schmitt, L.C., Rau, A., Seifert, O., Honer, J., Schmid, S., Hutt, M., Zantow, J., Hust, M., Dübel, S., Olayioye, M.A. and Kontermann, R.E. (2017). Inhibition of HER3 activation and tumor growth with a human antibody binding to a conserved epitope formed by domain III and IV. mAbs 9: 831-843
  • (111) Froude, J.W., Pelat, T., Miethe, S., Zak, S.E., Wec, A.Z., Chandran, K., Brannan, J.M., Bakken, R.R., Hust, M., Thullier, P. and Dye, J.M. (2017). Generation and characterization of protective antibodies to Marburg virus. mAbs 9: 696-703
  • (110) Frenzel, A., Schirmann, T., Dübel, S. and Hust, M. (2017). Entwicklung von therapeutischen Antikörpern im Reagenzglas. GIT Labor-Fachzeitschrift, Wiley, 4/2017: 29-32

  • (109) Noyan, F., Zimmermann, K., Hardtke-Wolenski, M., Knoefel, A., Schulde, E., Geffers, R., Hust, M., Huehn, J., Galla, M., Morgan, M., Jokuszies, A., Manns, M.P., Jaeckel, E. (2017). Prevention of allograft rejection by use of regulatory T cells with a MHC-specific chimeric antigen receptor. American Journal of Transplantation 17: 917-930


2016
  • (108) Postel, S., Deredge, D., Bonsor, D.A., Yu, X., Diederichs, K., Helmsing, S., Vromen, A., Friedler, A., Hust, M., Egelman, E.H., Beckett, D., Wintrode, P.L. and Sundberg, E.J. (2016). Bacterial flagellar capping proteins adopt diverse oligomeric states. eLIFE 5:e18857

  • (107) Zantow, J., Just, S., Lagkouvardos, I., Kisling, S., Dübel, S., Lepage, P., Clavel, T. and Hust, M. (2016). Mining gut microbiome oligopeptides by functional metaproteome display. Scientific Reports 6:34337
  • (106) Derman, Y., Selby, K., Miethe, S., Frenzel, A., Liu, Y., Rasetti-Escargueil, C., Avril, A., Pelat, T., Urbain, R., Fontayne, A., Thullier, P., Sesardic, D., Lindström, M., Hust, M. and Korkeala, H. (2016). Neutralization of Botulinum Neurotoxin Type E by a Humanized Antibody. Toxins 8: 257
  • (105) Miethe, S., Mazuet, C., Liu, Y., Tierney, R., Rasetti-Escargueil, C., Avril, A., Frenzel, A., Thullier, P., Pelat, T., Urbain, R., Fontayne, A., Sesardic, D., Hust, M. and Popoff, M.-R. (2016). Development of Germline-Humanized Antibodies Neutralizing Botulinum Neurotoxin A and B. PLoS ONE 11: e0161446

  • (104) Mendonça, M., Moreira, G.M., Conceição, F.R., Hust, M., Mendonça, K.S., Moreira, Â.N., França, R.C., da Silva, W.P., Bhunia, A.K. and Aleixo, J.A.. (2016). Fructose 1,6-Bisphosphate Aldolase, a Novel Immunogenic Surface Protein on Listeria Species. PLoS ONE 11(8): e0160544
  • (103) Frenzel, A., Schirrmann, T. and Hust, M. (2016). Phage display-derived human antibodies in clinical development and therapy. mAbs 8: 1177-1194
  • (102) Kuhn, P., Fühner, V., Moreira, G.M.S.G., Frenzel, A., Miethe, S. and Hust, M. (2016). Recombinant Antibodies for Diagnostics and Therapy against Pathogens and Toxins generated by Phage Display.  Proteomics - Clinical Applications 10: 922-948

  • (101) Zantow, J., Dübel, S. and Hust, M. (2016). Funktionales Proteom-Display zur Identifikation von Biomarkern. Biospektrum 22: 256-259

  • (100) Schieferdecker, A., Oberle, A., Thiele, B., Hofmann, F., Göthel, M, Miethe, S., Hust, M., Braig, F., Voigt, M., Koch-Nolte, F., Haag, F., Alawi, M., Indenbirken, D., Grundhoff, A., Bokemeyer, C., Bacher, U., Kröger, N. and Binder, M. (2016). A transplant immunome deep sequencing and screening platform defines a unique targetable epitope fingerprint of multiple myeloma. Blood 127: 3202-3214

  • (99) Rohrbeck, A., Fühner, V., Schröder, A., Hagemann, S., Vu, X.-H., Berndt, S., Hust, M., Pich, A. and Just, I. (2016). Detection and Quantification of ADP-Ribosylated RhoA/B by Monoclonal Antibody. Toxins 8: 100

  • (98) Blokzijl, A., Zieba, A., Hust, M., Schirrmann, T., Helmsing, S., Grannas, K., Hertz, E., Moren, A., Chen, L., Soderberg, O., Moustakas, A., Dübel, S. and Landegren, U. (2016). Single chain antibodies as tools to study TGF-β regulated SMAD proteins in proximity ligation-based pharmacological screens. Molecular&Cellular Proteomics 15: 1848-1856
  • (97) Connor, D.O., Zantow, J., Hust, M., Bier, F.F. and von Nickisch-Rosenegk, M. (2016). Identification of Novel Immunogenic Proteins of Neisseria gonorrhoeae by Phage Display. PLoS ONE 11(2): e0148986
  • (96) Kibat, J., Schirrmann, T., Knape, M.J., Helmsing, S., Meier, D., Hust, M., Schröder, C., Bertinetti, D., Pardes, K., Funk, M., Lages, A., Giese, N., Herberg, F. W., Dübel, S. and Hoheisel, J.D.  (2016). Utilisation of antibody microarrays for the selection of specific and informative antibodies from recombinant library binders of unknown quality. New Biotechnology 33: 574-581
  • (94) Unkauf, T., Miethe, S. Fühner, V., Schirrmann, T., Frenzel, A. and Hust, M. (2016). Generation of recombinant antibodies against toxins and viruses by phage display for Diagnostics and Therapy.  Advances in Experimental Medicine and Biology 917: 55-76

2015
  • (93) Rasetti-Escargueil, C., Avril, A., Tierney, R., Bak, N., Miethe, S., Chahboun, S., Mazuet, C., Popoff, M., Thullier, P., Hust, M., Pelat, T. and Sesardic, D. (2015). Development of human-like scFv-Fc neutralising Botulinum toxin type B. mAbs 6: 1161-1177
  • (92) Miethe, S., Rasetti-Escargueil, C., Avril, A., Liu, Y., Chahboun, S., Korkeala, H., Mazuet, C., Popoff, M.-R., Pelat, T., Thullier. P., Sesardic, D. and Hust, M. (2015).  Development of human-like scFv-Fc neutralizing botulinum neurotoxin E. PLoS ONE 10: e0139905
  • (91) Avril, A., Miethe, S., Popoff, M., Mazuet, C., Chahboun, S., Rassetti-Escargueil, C., Sesardic, D., Thullier, P., Hust, M. and Pelat, T. (2015). Isolation of nanomolar scFvs of non-human primate origin, cross-neutralizing botulinum neurotoxins A1 and A2 by targeting their heavy chain. BMC Biotechnology 15: 86
  • (90) Wirsing, L., Klawonn, F., Sassen, W., Lünsdorf, H., Probst, C., Hust, M., Mendel, R., Kruse, T. and Jänsch, L. (2015). Linear discriminant analysis identifies mitochondrially localized proteins in Neurospora crassa. Journal of Proteome Research 14: 3900-3911
  • (89) Droste, P., Frenzel, A., Steinwand, M., Pelat, T., Thullier, P., Hust, M., Lashuel, H. and Dübel, S. (2015). Structural differences of amyloid-β fibrils revealed by antibodies from phage display. BMC Biotechnology 15: 57
  • (88) Becker, M., Felsberger, A., Frenzel, A., Shattuck, W.M.C, Dyer, M., Kügler, J., Zantow, J., Mather, T. and Hust, M. (2015). Application of M13 Phage Display for Identifying Immunogenic Proteins from Tick (Ixodes scapularis) Saliva. BMC Biotechnology 15: 43
  • (87) Kügler, J., Wilke, S. Meier, D., Tomszak, F.,  Frenzel, A. , Schirrmann, T., Dübel, S.,  Garritsen, H.,  Hock, B., Toleikis, L., Schütte, M. and Hust, M. (2015). Generation and analysis of the improved human HAL9/10 antibody phage display libraries. BMC Biotechnology 15: 10


2014
  • (86) Froude, J.W., Pelat, T., Miethe, S., Zak, S., Bakken, R., Hust, M., Thullier, P. and Dye, J.M. (2014). A Multinational Consortium for the Development of Therapeutic Antibodies to Tier 1 Bioterrorism Agents: Protective scFv-Fc Antibody against Marburg Virus derived from Macaque Immune Library. STO Scientific Publications, STO-MP-HFM239-18
  • (85) Miethe, S., Pelat, T., Avril, A., Popoff, M., Molgo, J., Rasetti‑Escargueil, C., Fontayne, A., Korkeala, H., Thullier, P., Sesardic, D. and Hust, M. (2014). Development of recombinant antibodies for biodefense using phage display. STO Scientific Publications, STO-MP-HFM239-16
  • (84) Hust, M., Frenzel, A., Tomszak, F., Kügler, J. and Dübel, S. (2014). Antibody Phage Display. In: Handbook of therapeutic antibodies, 2nd ed., Ed. Dübel, S. and Reichert, J.M., Wiley-VCH, Weinheim, 43-76 (ISBN 978-3-527-32937-3)
  • (83) Wilke, S. and Hust, M. (2014). Cetuximab (Erbitux). In: Handbook of therapeutic antibodies, 2nd ed., Ed. Dübel, S. and Reichert, J.M., Wiley-VCH, Weinheim, 1501-1520 (ISBN 978-3-527-32937-3)
  • (82) Stech, M., Hust, M., Schulze, C., Dübel, S. and Kubick, S. (2014). Cell-free eukaryotic systems for the production, engineering and modification of scFv antibody fragments. Engineering in Life Sciences 14: 387-398
  • (81) Fuchs, M., Kämpfer, S., Helmsing, S., Spallek, R., Oehlmann, W., Prilop, W., Frank, R., Dübel, S., Mahavir, S. and Hust, M. (2014). Novel human recombinant antibodies against Mycobacterium tuberculosis antigen 85B. BMC Biotechnology 14: 68
  • (80) Marschall, A.L.J., Zhang, C., Frenzel, A., Schirrmann, T., Hust, M., Perez, F. and Dübel, S (2014). Delivery of antibodies to the cytosol - debunking the myths. mAbs 6: 943-956
  • (79) Trott, M., Weiß, S., Antoni, S., Koch, J., von Briesen, H., Hust, M. and Dietrich, U. (2014).  Functional characterization of two scFv-Fc antibodies from an HIV controller selected on soluble HIV-1 Env complexes: a neutralizing V3- and a trimer-specific gp41 antibody. PLoS ONE 9:  e97478
  • (78) Hülseweh, B., Rülker, T., Pelat, T., Langermann, C., Frenzel, A., Schirrmann, T., Dübel, S., Thullier, P. and Hust, M. (2014). Human-like antibodies neutralizing Western equine encephalitis virus. mAbs 6: 717-726
  • (77) Hust, M., Frenzel, A., Schirrmann, T. and Dübel, S. (2014). Selection of recombinant antibodies from antibody gene libraries. Gene Function Analysis, Ed: Ochs, M.F., Methods Molecular Biology 1101: 305-320
  • (76) Miethe, S., Rasetti-Escargueil, C., Liu, Y., Chahboun, S., Pelat, T., Avril, A., Frenzel, A., Schirrmann, T., Thullier, P., Sesardic, D. and Hust, M. (2014). Development of neutralizing scFv-Fc against botulinum neurotoxin A light chain from a macaque immune library. mAbs 6: 446-459
  • (75) Steinwand, M., Droste, P., Frenzel A., Hust, M., Dübel, S. and Schirrmann, T. (2014). The influence of antibody fragment format on phage display based affinity maturation of IgG. mAbs 6: 204-218
  • (74) Frenzel, A., Kügler, J., Wilke, S., Schirrmann, T. and Hust, M. (2014). Construction of human antibody gene libraries and selection of antibodies by phage display. Human Monoclonal Antibodies: Methods and Protocols, Ed: Steinitz, M., Methods Molecular Biology 1060: 215-243


2013
  • (73) Kügler, J., Zantow, J., Meyer, T., and Hust, M. (2013). Oligopeptide M13 Phage Display in Pathogen Research.  Viruses 5:2531-2545
  • (72) Frenzel, A., Hust, M. and Schirrmann, T. (2013). Expression of recombinant Antibodies. Frontiers in Immunology 4:217
  • (71) Jäger, V.,  Büssow, K., Wagner, A., Weber, S., Hust, M., Frenzel, A. and Schirrmann, T. (2013). High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells. BMC Biotechnology 13:52
  • (70) Zhou, M., Meyer, T., Koch, S., Koch, J., Brill, B., von Briesen, H., Benito, J.M., Soriano, V., Haberl, A., Bickel, M., Dübel, S., Hust, M. and Dietrich, U. (2013). Identification of an epitope in the membrane proximal external region of gp41 targeted by neutralizing antibodies in plasma from an elite controller using an Env-tailored phage display library. Eur J immunol 43: 499-509
  • (69) Miethe, S., Meyer, T., Wöhl-Bruhn, S.,  Frenzel. A., Schirrmann, T., Dübel, S. and Hust, M. (2013). Production of Single Chain Fragment Variable (scFv) Antibodies in Escherichia coli Using the LEX Bioreactor.  Journal of Biotechnology 163: 105-111 
  • (68) Bertz, A., Wöhl-Bruhn, S., Miethe, S., Tiersch, B., Koetz, J., Hust, M., Bunjes, H. and Menzel, H. (2013). Encapsulation of Proteins in Hydrogel Carrier Systems for Controlled Drug Delivery: Influence of Network Structure and Drug Size on Release Rate. Journal of Biotechnology 163: 243-249
  • (67) Al-Halabi, L., Balck, A., Michalzik, M., Fröde, D., Büttgenbach, S., Hust, M., Schirrmann, T. and Dübel, S. (2013). Recombinant antibody fragments allow repeated measurements of C-reactive protein with a quartz crystal microbalance immunosensor. mAbs 5: 140-149


2012
  • (66) Hust, M., Frenzel, A., Meyer, T., Schirrmann, T. and Dübel, S. (2012). Construction of human naive antibody gene libraries. Antibody Engineering: Methods and Protocols, Ed: Chames, P.,  Methods Molecular Biology 907: 85-107
  • (65) Wezler, X., Hust, M., Helmsing, S., Schirrmann, T. and Dübel, S. (2012). Human antibodies targeting CD30+ lymphomas. Human Antibodies 21:13-28
  • (64) Meyer, T.,  Schirrmann, T., Frenzel, A., Miethe, S., Stratmann-Selke, J., Gerlach, G.F., Strutzberg-Minder, K., Dübel, S., and Hust, M. (2012). Identification of immunogenic proteins and generation of antibodies against Salmonella Typhimurium using phage display. BMC Biotechnology 12:29
  • (63) Rülker, T., Voß, L., Thullier, P., O'Brien, L.M., Pelat, T., Perkins, S.D., Langemann, C., Schirrmann, T., Dübel, S., Marschall, H.-J., Hust, M. and Hülseweh, B. (2012). Isolation and characterisation of a human-like antibody fragment (scFv) that inactivates VEEV in vitro and in vivo. PLoS ONE 7: e37242
  • (62) Frenzel, A., Fröde, D., Meyer, T., Schirrmann, T. and Hust, M. (2012). Generating recombinant antibodies for research, diagnostics and therapy using phage display. Current Biotechnology 1:33-41
  • (61) Zhang, C., Helmsing, S., Zagrebelsky, M., Schirrmann, T., Marschall, A.L.J., Schüngel, M., Korte, M., Hust, M. and Dübel, S. (2012). Suppression of p75 Neurotrophin Receptor Surface Expression with Intrabodies Influences Bcl-xL mRNA Expression and Neurite Outgrowth in PC12 Cells. PLoS ONE 7: e30684


2011
  • (60) David, F., Steinwand, M., Hust, M., Bohle, K., Ross, A., Dübel, S. and Franco-Lara, E. (2011). Antibody production in Bacillus megaterium: strategies and physiological implications of scaling from micro titer plates to industrial bioreactors. Biotechnology Journal 6:1516-1531
  • (59) Thullier, P., Hülseweh, B., Pelat, T., Rülker, T., Miethe, S., Dübel, S. and Hust, M. (2011). Development of human and macaque antibodies using antibody phage display for the detection of equine encephalitis viruses. In: Encephalitis / Book 3, Ed: Hayasaka, D., Intech, Rijeka, 309-322 (978-953-307-741-3)
  • (58) Chahboun, S., Hust, M., Liu, Y., Pelat, T., Miethe, S., Helmsing, S., Jones ,R.G., Sesardic, D. and Thullier, P. (2011). Isolation of a nanomolar scFv inhibiting the endopeptidase activity of botulinum toxin A, by single-round panning of an immune phage-displayed library of macaque origin. BMC Biotechnology 11:113
  • (57) Colwill, K., Persson, H., Jarvik, N.E., Wyrzucki, A., Wojcik, J., Koide, A., Kossiakoff, A.A., Koide, S., Sidhu, S., Dyson, M.R., Pershad, K., Pavlovic, J.D., Karatt-Vellat, A., Schofield, D.J., Kay, B.K., McCafferty, J., Mersmann, M., Meier, D., Mersmann, J., Helmsing, S., Hust, M., Dübel, S., Berkowicz, S., Freemantle, A., Spiegel, M., Sawyer, A., Layton, D., Nice, E., Dai, A., Rocks, O., Williton, K., Fellhouse, F.A., Hersi, K., Pawson, T., Nilsson, P., Sundberg, M., Sjöberg, R., Sivertsson, A., Schwenk, J.M., Takanen, J.O., Hober, S., Uhlén, M., Dahlgren, L.G., Flores, A. Johansson, I, Weigelt, J., Crombet, L., Loppnau, P., Kozieradzki, I., Cossar, D., Arrowsmith, C.H., Edwards, A.M. and Gräslund, S. (2011). A roadmap to generate renewable protein binders to the human proteome. Nature Methods 8, 551-558

  • (56) Lüders, S., David, F., Steinwand, M., Jordan, E., Hust, M., Dübel, S. and Franco-Lara, E. (2011). Influence of the hydromechanical stress and temperature on growth and antibody fragment production with Bacillus megaterium. Applied Microbiology and Biotechnology 91, 81-90

  • (55) Hust, M., Meyer, T., Voedisch, B., Rülker, T., Thie, H., El-Ghezal, A., Kirsch, M.I., Schütte, M., Helmsing, S., Meier, D., Schirrmann, T. and Dübel, S. (2011). A human scFv antibody generation pipeline for proteome research. Journal of Biotechnology 152, 159-170
  • (54) Dammeyer, T., Steinwand, M., Krüger, S.C., Dübel, S., Hust, M. and Timmis, K.N. (2011). Efficient production of soluble recombinant single chain Fv fragments by a Pseudomonas putida strain KT2440 cell factory. Microbial Cell Factories 10: 11
  • (53) Thie, H., Toleikis, L., Li, J., von Wasielewski, R., Bastert, G., Schirrmann, T., Esteves, I. T., Behrens, C. K.,  Fournes, B., Fournier, N., de Romeuf, C., Hust, M. and Dübel, S. (2011). Rise and fall of an anti-MUC1 specific antibody. PLoS ONE 6: e15921

  • (52) Schirrmann, T., Meyer, T., Schütte, M., Frenzel, A., and Hust, M. (2011). Phage display for the generation of antibodies for proteome research, diagnostics and therapy. Molecules 16, 412-426
  • (51) Meyer, T.,  Stratmann-Selke, J., Meens, J., Schirrmann, T., Gerlach G.F., Frank, R., Dübel, S., Strutzberg-Minder, K. and Hust, M (2011). Isolation of scFv fragments specific to OmpD of Salmonella Typhimurium. Veterinary Microbiology 147, 162-169

2010
  • (50) Bourbeillon, J., Orchard, S., Benhar, I., Borrebaeck, C., de Daruvar, A., Dübel, S., Frank, R., Gibson, F., Gloriam, D., Haslam, N., Hiltker, T., Humphrey-Smith, I., Hust, M., Juncker, D., Koegl, M., Konthur, Z., Korn, B., Krobitsch, S., Muyldermans, S., Nygren, P.-A., Palcy, S., Polic, B., Rodriguez, H., Sawyer, A., Schlapshy, M., Snyder, M.,Stoevesandt, O., Taussig, M.J., Templin, M., Uhlen, M., van der Maarel, S., Wingren, C., Hermjakob, H. and Sherman, D. (2010). Minimum Information about a Protein Affinity Reagent (MIAPAR). Nature Biotechnology 28, 650-653
  • (49) Schirrmann, T. and Hust, M. (2010). Construction of human antibody gene libraries and selection of antibodies by phage display. Immunotherapy of Cancer: Methods and Protocols, Ed. Yotnda, P.,  Methods Molecular Biology 651, 177-209
  • (48) Dübel, S., Stoevesandt, O., Taussig, M.J. and Hust, M. (2010). Generating recombinant antibodies to the complete human proteome. Trends in Biotechnology 28, 333-339
  • (47) Naseem, S., Meens, J., Jores, J., Heller, M., Dübel, S., Hust, M. and Gerlach, G.F. (2010). Phage display-based identification and potential diagnostic application of novel antigens from Mycoplasma mycoides subsp. mycoides small colony type. Veterinary Microbiology 142, 285-292
  • (46) Steinwand, M., Jordan, S. and Hust, M. (2010). Production of Antibody Fragments in the Gram-positive bacterium B. megaterium. In: Antibody engineering Vol 2, Ed: Kontermann, R. and Dübel, S., Springer-Verlag, Berlin Heidelberg, 293-299 (ISBN 978-3-642-01143-6)
  • (45) Mollova, S., Retter, I., Hust, M., Dübel, S. and Müller, W. (2010). Analysis of single chain antibody sequences using the VBASE2 Fab Analysis Tool. In: Antibody engineering Vol 2, Ed: Kontermann, R. and Dübel, S., Springer-Verlag, Berlin Heidelberg, 3-10 (ISBN 978-3-642-01143-6)
  • (44) Breitling, F., Broders, O., Helmsing, S., Hust, M. and Dübel, S. (2010). Improving phage display throughput by using Hyperphage, miniaturized titration and pVIII (g8p) ELISA. In: Antibody engineering Vol 1, Ed: Kontermann, R. and Dübel, S., Springer-Verlag, Berlin Heidelberg, 197-206 (ISBN 978-3-642-01143-6)
  • (43) Hust, M. and Mersmann, M. (2010). Phage Display and selection in microtitre plates. In: Antibody engineering Vol 1, Ed: Kontermann, R. and Dübel, S., Springer-Verlag, Berlin Heidelberg, 139-150 (ISBN 978-3-642-01143-6)
  • (42) Pelat, T., Hust, M. and Thullier, P. (2010). Immune libraries from non-human primates (NHP). In: Antibody engineering Vol 1, Ed: Kontermann, R. and Dübel, S., Springer-Verlag, Berlin Heidelberg, 99-114 (ISBN 978-3-642-01143-6)
  • (41) Hust, M. and Dübel, S. (2010). Human antibody gene libraries. In: Antibody engineering, Ed: Kontermann, R. and Dübel, S., Springer-Verlag, Berlin Heidelberg, 65-84 (ISBN 978-3-642-01143-6)
  • (40) Mersmann, M., Meier, D., Mersmann, J., Helmsing, S., Nilsson, P., Gräslund S.,  Structural Genomics Consortium, Colwill, K., Hust, M. and Dübel, S. (2010). Towards "proteome scale" antibody selections using phage display. New Biotechnology 27, 118-128
  • (39) Schirrmann, T., Menzel, C., Hust, M., Prilop, J., Jostock, T. and Dübel, S. (2010). Oligomeric forms of single chain immunoglobulin (scIgG). mAbs 2, 73-76

2009

  • (38) Pelat, T., Hust., M. and Thullier, P. (2009). Obtention and engineering of non-human primate (NHP) antibodies for therapeutics. Mini Reviews in Medicinal Chemistry 14, 1633-1638
  • (37) Thie, H., Binius, S., Schirrmann, T., Hust, M. and Dübel, S. (2009). Multimerization domains for antibody phage display and antibody production. New Biotechnology 26, 314-321
  • (36) Hust, M., Steinwand, M., Al-Halabi, L., Helmsing, S., Schirrmann, T. and Dübel, S. (2009). Improved microtitre plate production of single chain Fv fragments in Escherichia coli. New Biotechnology 25, 424-428
  • (35) Schütte, M., Thullier, P., Pelat, T., Wezler, X., Rosenstock, P., Hinz, D., Kirsch, M.I., Hasenberg, M., Frank, R., Schirrmann, T., Gunzer, M., Hust, M. and Dübel, S. (2009). Identification of a putative Crf splice variant and generation of recombinant antibodies for the specific detection of Aspergillus fumigatus. PLoS ONE 4:e6625
  • (34) Pelat, T., Hust, M., Hale, M., Lefranc, M.-P., Dübel, S. and Thullier, P. (2009). Isolation of a human-like antibody fragment (scFv) that neutralizes ricin biological activity. BMC Biotechnology 8:66
  • (33) Hust, M., Thie, H., Schirrmann, T. and Dübel, S. (2009). Antibody Phage Display. In: Handbook of Therapeutic Monoclonal Antibodies: From Bench to Clinic, Ed: An, Z. and Strohl, W., Wiley-VCH , 191-212
  • (32) Jordan, E., Al-Halabi, L., Schirrmann, T. and Hust, M. (2009). Antibody production by the Gram-positive bacterium Bacillus megaterium. Therapeutic Antibodies, Ed: Dimitrov, A.S.,  Methods Molecular Biology 525, 509-516
  • (31) Thie, H., Voedisch, B., Dübel, S., Hust, M. and Schirrmann, T. (2009). Affinity maturation by phage display. Therapeutic Antibodies, Ed: Dimitrov, A.S.,  Methods Molecular Biology 525, 309-322
  • (30) Strebe, N., Guse, A., Schüngel, M., Schirrmann, T., Haffner, M., Jostock, T., Hust, M., Müller, W. and Dübel, S. (2009). Fuctional knockdown of VCAM-1 at the posttranslational level with ER retained antibodies. Journal of immunological Methods 341, 30-40

2008
  • (28) Kirsch, M.I., Hülseweh, B., Nacke, C., Rülker, T., Schirrmann, T., Marschall, H.-J., Hust, M. and Dübel, S. (2008). Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV).  BMC Biotechnology 8:66
  • (27) Kügler, J., Nieswandt, S., Gerlach, G.F., Meens, J., Schirrmann, T. and Hust, M. (2008). Identification of immunogenic polypeptides from a Mycoplasma hyopneumoniae genome library by phage display. Applied Microbiology and Biotechnology 80, 447- 458
  • (26) Thie, H., Schirrmann, T., Paschke, M., Dübel, S. and Hust, M. (2008). SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coli. New Biotechnology 25, 49-54
  • (25) Schirrmann, T., Al-Halabi, L., Dübel, S. and Hust, M. (2008). Production systems for recombinant antibodies. Frontiers in Bioscience 13, 4576-4594
  • (24) Hust, M., Toleikis, L. and Dübel, S. (2007). Selection Strategies II: Antibody phage display. In: Handbook of therapeutic antibodies, Ed. Dübel, S., Wiley-VCH, 45-68

2007
  • (23) Michalzik, M., Balck, A., Al-Halabi, L., Hust, M., Dübel, S. and Büttgenbach, S. (2007). Massensensitives Sensor-Fließsystem zur CRP-Diagnostik. Mikrosystemtechnikkongress 2007, 939-942
  • (22) Schirrmann, T., Hust, M. and Dübel, S. (2007). Die Antikörperfabrik: Antikörper für jedes Protein. Biologie in unserer Zeit 37, 348-351
  • (21) Jordan, E., Al-Halabi, L., Schirrmann, T., Hust, M. and Dübel, S. (2007). Production of single chain Fab (scFab) in Bacillus megaterium.  Microbial Cell Factories 6:38
  • (20) Hust, M., Dübel, S. and Schirrmann, T. (2007). Selection of recombinant antibodies from antibody gene libraries. Gene Function Analysis, Ed: Ochs, M.F.,  Methods Molecular Biology 408, 241-254
  • (19) Pelat, T., Hust, M., Laffly. E.,  Condemine, F., Bottex, C., Vidal, D., Lefranc, M.P., Dübel, S. and Thullier, P. (2007). A high affinity, human like antibody fragment (scFv) neutralising lethal factor (LF) of Bacillus anthracis by inhibiting the PA-LF complex formation. Antimicrobial agents and chemotherapy 51, 2758-2764
  • (18) Schirrmann, T., Hust, M., Konthur, Z., Frank, R., Toleikis, L. and Dübel, S. (2007). Schlüsselreagenzien für die Proteomforschung im Hochdurchsatz. Laborwelt 2/2007, 16-20
  • (17) Hust, M., Jostock, T., Menzel, C., Voedisch, B., Mohr, A., Brenneis, M., Kirsch, M.I., Meier, D. and Dübel, S. (2007). Single chain (scFab) fragment. BMC Biotechnology 7:14
  • (16) Jordan, E., Hust, M., Roth, A., Biedendieck, R., Schirrmann, T., Jahn, D. and Dübel, S. (2007). Production of recombinant antibody fragments in Bacillus megaterium. Microbial Cell Factories 6:2
  • (15) Soltes, G., Hust, M., Ng, K.K.Y., Bansal, A., Field, J., Stewart, D.I.H., Dübel, S., Cha, S. and Wiersma, E. (2007). On the influence of vector design on antibody phage display. Journal Biotechnology 127, 626-637

2006
  • (14) Michalzik, M., Balck, A., Büttgenbach, S., Al-Halabi, L., Hust, M. and Dübel, S. (2006). Microsensor System for Biochemical and Medical Analysis. Proc. XX Eurosensors, 420-421
  • (13) Hust, M., Meysing, M, Schirrmann, T., Selke, M., Meens, J., Gerlach, G.-F. and Dübel, S. (2006). Enrichment of open reading frames presented on bacteriophage M13 using Hyperphage. Biotechniques 41, 335-342

2005
  • (12) Konthur, Z., Hust, M. and Dübel, S. (2005). Perspectives for systematic in vitro antibody generation. Gene 364, 19-29
  • (11) Voedisch, B., Menzel. C., Jordan, E., El-Ghezal, A., Schirrmann, T., Hust, M. und Jostock, T. (2005). Heterologe Expression von rekombinanten Proteinpharmazeutika. Laborwelt 3/2005, 31-35
  • (10) Hust, M., Toleikis, L. and Dübel, S. (2005). Antibody Phage Display. Mod. Asp. Immunobiol. 15, 47-49
  • (9) Voedisch, B., Menzel. C., Jordan, E., El-Ghezal, A., Schirrmann, T., Hust, M. und Jostock, T. (2005). Expression rekombinanter Proteinpharmazeutika. transkript 7/2005, 47-51
  • (8) Kirsch, M., Zaman, M., Meier, D., Dübel, S. and Hust, M. (2005). Parameters affecting the display of antibodies on phage. Journal of Immunological Methods 301, 173-185
  • (7) Schirrmann, T., Hust, M. und Dübel, S. (2005). Schlüsselreagenzien für den nächsten großen Schritt. GenomXpress 2.05, 11-13
  • (6) Hust, M. and Dübel, S. (2005). Phage display vectors for the in vitro generation of human antibody fragments. Immunochemical Protocols, 3rd ed., Ed: Burns, R., Methods Molecular Biology 295, 71-95


2004
  • (5) Hust, M. and Dübel, S. (2004). Mating antibody phage display with proteomics. Trends in Biotechnology 22, 8-14


2003
  • (4) Meyer, T., Hust, M., Marquardt, J., Krumbein, W.E. and Rhiel, E. (2003). A methodological approach to investigate steady state fucoxanthin chlorophyll a/c binding protein mRNA levels in Wadden Sea sediments. International Microbiology 6, 33-39


2002
  • (3) Hust, M., Maiss, E., Jacobsen, H.-J. and Reinard, T. (2002). The production of a genus specific recombinant antibody (scFv) using a recombinant Potyvirus protease. Journal of Virological Methods 106, 225-233


1999
  • (2) Hust, M., Krumbein, W.E. and Rhiel, E. (1999). An immunochemical in situ approach to detect adaptation processes in the photosynthetic apparatus of diatoms of the Wadden Sea sediment surface layers. Journal of Microbiological Methods 38, 69-80

  • (1) Janssen, M., Hust, M., Rhiel, E. and Krumbein, W.E. (1999). Vertical migration behaviour of diatom assemblages of Wadden Sea sediments (Dangast/Germany): A study using Cryo-scanning electron microscopy. International Microbiology 2, 103-110


Editorial Activities


Other Activities

  • Expertenpools des Nationalen Ausschuss für den Schutz von für wissenschaftliche Zwecke verwendeten Tieren
  • Scientific Coordinator of the EC consortium AntibotABE (-2015)
  • Member of Deutscher Hochschulverband (DHV)
  • Member of Verband Biologie, Biowissenschaften und Biomedizin in Deutschland (VBIO)
  • Co-founder of mAb-factory GmbH


PubMed entries of Michael

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ORCID ORCID (Original Citation ID)



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